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Dietary phospholipids are more efficient than neutral lipids for long-chain polyunsaturated fatty acid supply in European sea bass Dicentrarchus labrax larval development ArchiMer
Gisbert, Enric; Villeneuve, Laure; Zambonino, Jose-luis; Quazuguel, Patrick; Cahu, Chantal.
We evaluated the effects of dietary lipid class (phospholipid vs. neutral lipid) and level of n-3 long-chain PUFA (LC-PUFA) on the growth, digestive enzymatic activity, and histological organization of the intestine and liver in European sea bass larvae. Fish were fed from the onset of exogenous feeding at 7 to 37 d post-hatch with five isoproteic and isolipidic compound diets with different levels of EPA and DHA. Diet names indicated the percentage of EPA and DHA contained in the phospholipids (PL) and neutral lipids (NL), as follows: PL5, PL3, PL1, NL1, and NL3. Histological observations showed different patterns of lipid absorption and accumulation in the intestinal mucosa depending on the level and nature of the dietary lipid fraction. Fish fed high...
Tipo: Text Palavras-chave: Histology; Digestive system; Phospholipid; Larvae; Dicentrarchus labrax; Dietary.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-929.pdf
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Effect of nature of dietary lipids on European sea bass morphogenesis: implication of retinoid receptors ArchiMer
Villeneuve, Laure; Gisbert, Enric; Zambonino, Jose-luis; Quazuguel, Patrick; Cahu, Chantal.
The effect of the nature and form of supply of dietary lipids on larval development was investigated in European sea bass larvae, by considering the expression of several genes involved in morphogenesis. Fish were fed from 7 to 37 d post-hatch with five isoproteic and isolipidic compound diets incorporating different levels of EPA and DHA provided by phospholipid or neutral lipid. Phospholipid fraction containing 1 center dot 1 % (PL1 diet) to 2 center dot 3 % (PL3 diet) of EPA and DHA sustained good larval growth and survival, with low vertebral and cephalic deformities. Similar levels of EPA and DHA provided by the neutral lipid fraction were teratogenic and lethal. Nevertheless, dietary phospholipids containing high levels of DHA and EPA (PL5 diet)...
Tipo: Text Palavras-chave: Retinoic acid receptors; Skeletal malformations; Dicentrarchus labrax larvae; Neutral lipids; Phospholipids.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-880.pdf
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Influence nutritionnelle de la vitamine A et de la nature des lipides sur la morphogenèse de la larve de bar (Dicentrarchus labrax): implicaton de la voie des rétinoïdes ArchiMer
Villeneuve, Laure.
The aim of this study was to determine the nutritional effects of vitamin A and PUFA (including EPA and DHA) on sea bass larvae development. An excess of vitamin A in the diet led to malformations affecting the cephalic region linked to an increase of RARgama transcription and the loss of a vertebra when larvae were fed this diet from 8 days post hatch (dph) to 13 dph. A diet containing an excess of PUFA disrupted the development of larvae vertebral column, deformities were correlated to a decrease of RARalpha, RARgama, RXRalpha and BMP4 transcription and an additional vertebra appeared when larvae were fed this diet from 8 dph to 13 dph. A diet containing 31 mg/kg of dry matter of vitamin A and between 1.1 and 2.3% of EPA + DHA allows larvae to develop...
Tipo: Text Palavras-chave: Malformations; Genes of development; PUFA; Vitamin A; Sea bass Dicentrarchus labrax; Larvae; Malformations; Gènes du développement; AGPI; Vitamine A; Bar Dicentrarchus labrax; Larves.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/these-324.pdf
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Intake of high levels of vitamin A and polyunsaturated fatty acids during different developmental periods modifies the expression of morphogenesis genes in European sea bass (Dicentrarchus labrax) ArchiMer
Villeneuve, Laure; Gisbert, Enric; Moriceau, Jacques; Cahu, Chantal; Zambonino, Jose-luis.
The effect of the feeding period on larval development was investigated in European sea bass larvae by considering the expression level of some genes involved in morphogenesis. Larvae were fed a control diet except during three different periods (period A: from 8 to 13 d post-hatching (dph); period B: from 13 to 18 dph; period C: from 18 to 23 dph) with two compound diets containing high levels of vitamin A or PUFA. European sea bass morphogenesis was affected by these two dietary nutrients during the early stages of development. The genes involved in morphogenesis could be modulated between 8 and 13 dph, and our results indicated that retinoids and fatty acids influenced two different molecular pathways that in turn implicated two different gene cascades,...
Tipo: Text Palavras-chave: Retinoid pathway; Morphogenesis; Vitamin A; PUFA; Sea bass larvae.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1215.pdf
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